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激光与光电子学进展, 2023, 60 (15): 1530003, 网络出版: 2023-08-11 低温胁迫对谷物种子萌发影响的红外光谱研究 下载： 612次【增强内容出版】Effects of Low-Temperature Stress on Grain Seeds Germination by Infrared SpectroscopyGet PDFFull Text图表MetricsMore 车前 1刘刚 1,*田雪 1欧全宏 1严伟敏 1时有明 2
作者单位 1 云南师范大学物理与电子信息学院，云南 昆明 6505002 曲靖师范学院物理与电子信息学院，云南 曲靖 655011
光谱学 谷物种子 低温胁迫 红外光谱 曲线拟合 spectroscopy grain seeds low-temperature stress infrared spectroscopy curve fitting AI词云图 AI语音精读 AI语音超短摘要【AI生成本文一句话精读】：低温胁迫对谷物种子萌发有显著影响，红外光谱研究揭示了种子内部化学变化，有助于理解低温对种子生理的影响机制。【AI生成本文短摘要】：低温胁迫对谷物种子萌发有显著影响，利用红外光谱技术研究发现，低温改变了种子内部蛋白质和多糖的结构，影响了萌发能力。该技术为深入了解低温对种子生理生化的影响提供了新的视角，有助于优化农作物抗寒育种策略，保障粮食安全。注：本部分内容由 AI 自动生成，仅供您参考。对于您使用本站 AI 自动生成内容所产生的一切后果，本网站及平台运营方概不承担任何商业和法律责任，请您知悉。 摘要种子萌发作为植物生命历程的开端，直接影响作物的最终产量。种子萌发常遭遇低温的影响，严重威胁粮食生产安全。利用傅里叶变换红外光谱结合曲线拟合研究低温对谷物种子（藜麦、青稞、水稻和小麦）萌发的影响。研究结果显示，4种谷物种子的发芽势、发芽率和发芽指数都随温度的降低而下降，青稞种子在4 ℃低温下的发芽率和发芽指数仍然较高，表明青稞种子的耐低温能力更强。红外光谱结果显示，低温胁迫下的谷物种子原始红外光谱整体特征相似，主要由多糖、脂肪和蛋白质的特征吸收峰组成。对低温胁迫下的谷物种子多糖（1200~950 cm-1）和酰胺Ⅰ带（1700~1600 cm-1）进行曲线拟合，结果显示：藜麦种子的多糖含量上升，蛋白质含量下降；青稞种子的多糖和蛋白质含量均呈先升后降的趋势；水稻种子的多糖含量下降，蛋白质含量上升；小麦种子的多糖含量呈先升后降的趋势，蛋白质含量呈先降后升的趋势。另外，在低温胁迫下，藜麦、青稞、水稻和小麦种子中都有不同比例的蛋白质二级结构从无序转向有序。因此，傅里叶变换红外光谱结合曲线拟合是一种研究低温胁迫对种子萌发影响的有效方法。 AbstractAs the beginning of the plant life process, seed germination directly affects the final crop yield. Low-temperature frequently inhibit seed germination, posing a serious threat to food production safety. The effects of low-temperature on the germination of grain seeds (quinoa, highland barley, rice, and wheat) were investigated in this paper using Fourier transform infrared spectroscopy combined with curve-fitting. The results showed that the germination potential, germination rate, and germination index of the four grain seeds decreased as the temperature decreased, but the germination rate and germination index of highland barley seeds remained high at 4 ℃, indicating that highland barley seeds had stronger low-temperature tolerance. The results of infrared spectrum demonstrated that the original infrared spectra of grain seeds under low-temperature were similar, mainly composed of the characteristic absorption peaks of polysaccharide, fat, and protein. Under low-temperature stress, the curve fitting analysis for polysaccharide (1200-950 cm-1) and amide Ⅰ band (1700-1600 cm-1) in quinoa seeds revealed that polysaccharide content increased while protein content decreased. The polysaccharide and protein content of highland barley seeds increased at first, then decreased. The content of polysaccharide in rice seed decreased, while the content of protein increased. The polysaccharide content of wheat seeds increased first and then decreased, while the protein content decreased first and then increased. Furthermore, under low-temperature stress, different proportions of protein secondary structures in quinoa, highland barley, rice, and wheat seeds changed from disorder to order. As a result, Fourier transforms infrared spectroscopy combined with curve fitting is an efficient method for investigating the effect of low-temperature stress on seed germination. PDF全文 车前, 刘刚, 田雪, 欧全宏, 严伟敏, 时有明. 低温胁迫对谷物种子萌发影响的红外光谱研究[J]. 激光与光电子学进展, 2023, 60(15): 1530003. Qian Che, Gang Liu, Xue Tian, Quanhong Ou, Weimin Yan, Youming Shi. Effects of Low-Temperature Stress on Grain Seeds Germination by Infrared Spectroscopy[J]. Laser & Optoelectronics Progress, 2023, 60(15): 1530003.
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