MPD: GMC02: project protocol

2023-10-08 00:44| 来源: 网络整理| 查看: 265

GMC02 project protocol Grip strength in 8 inbred founder strains of the Collaborative Cross (2020) German Mouse Clinic and Department of Infection Genetics, HZI With: Hrab臎 de Angelis M, Fuchs H, Gailus-Durner V, Becker L, Klopstock T, Lengger C, Kollmus H, Schughart K

German Mouse Clinic: Phenotyping Pipeline

Grip strength test: 8-10 wks of age

Project protocol - Contents Workflow and sampling Equipment and supplies Reagents and solutions Procedure: Grip strength test Data Workflow and sampling Step Procedure Equipment Data collected 1 Forelimb measurement Grip strength meter Grip strength over 3 trials 2 Forelimb/hindlimb measurement Grip strength meter Grip strength over 3 trials 3 Body weight measurement Balance Body weight Equipment and supplies Grip strength meter: Bioseb (Bio-GT3), FRANCE Balance: Ohaus (AV213C) Reagents and solutions Cleaning solution: 50% ethanol Procedure: Grip strength test The grip strength meter is calibrated according to manufacturer's instructions. Mice are removed individually from their homecage by gripping the base of the tail between the thumb and forefinger. Forelimb measurement: Each mouse is lowered over the grid keeping the torso horizontal and allowing only the forepaws to attach to the grid. The mouse is gently pulled back by its tail ensuring the mouse grips the top portion of the grid and the torso remains horizontal. The maximal grip strength is recorded. This step is repeated 2 more times to give a total of 3 trials. Forelimb/hindlimb measurement: Each mouse is lowered over the grid keeping the torso parallel with the grid and allowing all 4 paws to attach to the grid. The mouse is gently pulled back by its tail ensuring the torso remains parallel with the grid. Maximal grip strength is recorded. This step is repeated 2 more times to give a total of 3 trials. Body weight is measured so that grip strength can be normalized for each mouse. Mouse is returned to its homecage. Data collected by investigator Forelimb strength Forelimb/hindlimb strength Body weight

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