超氧化物歧化酶(SOD)活性检测试剂盒(ab65354/K335 | 您所在的位置:网站首页 › 试剂盒测定sod活性的方法 › 超氧化物歧化酶(SOD)活性检测试剂盒(ab65354/K335 |
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The ab65354 Superoxide Dismutase Activity Colorimetric Assay Kit can be used without a standard since it is reporting as % inhibition in your sample. Blank 1 does not contain any inhibition, and your sample will have some percentage of inhibition. Blank 2 and Blank 3 are background values so remember to minus these values when doing the calculation. Thus, the more SOD in a sample the less WST-1 formazan produced (which was dependent on Xanthine Oxidase (XO) activity). If you want to measure the amount of SOD in your sample, you can use a purified protein as a standard (ab112193 SOD full length protein) and then treat the standard just as you would treat a sample and calculate the amount using a standard curve. Here are the details of the standard. For your information, a 96-well plate is also not included in the kit but you can use a standard flat bottom plate such as the one sold by Greiner Bio one (#655-101) or any other standard polystyrene plate. Read More達偉 鄭 Abcam Scientific Support回复于 Jan 20 2014 |
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