研究开发出用于癌症成像治疗一体化的新型DNA

南京大学化学化工学院朱俊杰研究组开发出了一种用于癌症成像治疗一体化的新型DNA-金纳米机器。相关论文于2020年12月02日发表在《德国应用化学》杂志上。

在该研究中，课题组开发了一种连接核酸适配体的DNA-金（Apt-DNA-Au）纳米颗粒，以用于乳腺癌的原位成像和靶向多模式协同治疗。在被乳腺癌细胞MCF-7选择性内吞后，该Apt-DNA-Au纳米颗粒可通过DNA链置换被肿瘤相关的TK1 mRNA活化，释放出荧光分子和反义DNA，并导致金纳米颗粒团聚，以分别达到原位成像、抑制肿瘤相关蛋白survivin的表达和光热疗法的目的。

与此同时，控制释放的药物可产生化疗效果，而负载的光敏剂则可在激光照射下产生活性氧（ROS）用于光动力疗法。实验结果表明，这种Apt-DNA-Au纳米机器为原位成像引导的抗肿瘤联合疗法提供了一个强有力的纳米诊疗平台。

据了解，整合诊断和治疗功能的多功能纳米诊疗平台一直是癌症精确治疗中的棘手挑战。

附：英文原文

Title: Endogenous mRNA Triggered DNA‐Au Nanomachine for in Situ Imaging and Targeted Multimodal Synergistic Cancer Therapy

Author: Sha Yu, Yang Zhou, Yao Sun, Shaojun Wu, Tingting Xu, Yu-Chuang Cheng, Sai Bi, Li-Ping Jiang, Junjie Zhu

Issue&Volume: 02 December 2020

Abstract: Development of versatile nanotheranostic platforms that integrate both diagnostic and therapeutic functions have always been an intractable challenge in precise cancer treatment. Herein, an aptamer‐tethered deoxyribonucleic acids‐gold particle (Apt‐DNA‐Au) nanomachine has been developed for in situ imaging and targeted multimodal synergistic therapy of mammary carcinoma. Upon specifically internalized into MCF‐7 cells, the tumor‐related TK1 mRNA activates the Apt‐DNA‐Au nanomachine via DNA strand displacement cascades, resulting in the release of fluorophore and antisense DNA as well as the aggregation of AuNPs for in situ imaging, suppression of survivin expression and photothermal therapy, respectively. Meanwhile, the controlled released drugs are used for chemotherapy, while under the laser irradiation the loaded photosensitizer produces reactive oxygen species (ROS) for photodynamic therapy. The results confirm that the proposed Apt‐DNA‐Au nanomachine provides a powerful nanotheranostic platform for in situ imaging‐guided combinatorial anticancer therapy.

DOI: 10.1002/anie.202012801

Source: https://www.onlinelibrary.wiley.com/doi/10.1002/anie.202012801

Angewandte Chemie：《德国应用化学》，创刊于1887年。隶属于德国化学会，最新IF：12.959 官方网址：https://onlinelibrary.wiley.com/journal/15213773 投稿链接：https://www.editorialmanager.com/anie/default.aspx