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水稻护颖外稃化突变体lsl的遗传分析与基因定位
于洋, 杜丹, 张长伟, 杨正林, 凌英华, 何光华, 李云峰*
西南大学水稻研究所, 转基因植物与安全控制重庆市级重点实验室, 重庆400716
通信作者：李云峰；E-mail: [email protected]；Tel: 023-68251950 摘 要： 护颖是水稻等稻族禾本科植物的小穗器官, 关于其进化及发育的分子机制还不十分清楚。本研究报道了一个来源于保持系水稻‘西农1B’的EMS诱变群体中的护颖异常突变体, 暂被命名为lsl (lemma-like sterile lemma), 主要表现为护颖显著伸长变宽。花器官发育早期, lsl突变体护颖原基表现出与野生型护颖一致的发育分化模式; 到成熟期, lsl突变体护颖表层细胞严重硅化, 着生大量毛刺, 与外稃类似, 护颖内部细胞结构解剖也和外稃一致。这些结果表明突变体的护颖发生了向外稃的同源异型转化。将lsl与‘缙恢10号’杂交构建F1和F2定位群体。遗传分析表明该突变体受隐性单基因控制。利用SSR分子标记, 通过BSA法, 将LSL基因定位在第7染色体上SSR标记RM1085和RM6663之间, 物理距离为735.5 kb。本研究为进一步理解LSL基因的功能及分子机理奠定了良好的基础。关键词：水稻; 小穗; 不育外稃; 护颖外稃化突变体; 遗传分析 收稿：2015-03-25 修定：2015-07-14 资助：重庆市能力提升项目(cstc2014pt-sy80001)和攻关项目(cstc2012ggB80005)。 Genetic Analysis and Gene Mapping of a lemma-like sterile lemma (lsl) Mutant in Rice YU Yang, DU Dan, ZHANG Chang-Wei, YANG Zheng-Lin, LING Ying-Hua, HE Guang-Hua, LI Yun-Feng* Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute, Southwest University, Chongqing 400716, ChinaCorresponding author: LI Yun-Feng; E-mail: [email protected]; Tel: 023-68251950 Abstract: Rice is the model plant of Gramineae, and the sterile lemmas are its unique spikelet organs. The molecular mechanism of sterile lemma development doesn’t have a definite peroration. In this study, a sterile lemma mutant lsl (lemma-like sterile lemma) with significantly lemma-like, which is derived from ethyl methane sulfonate (EMS)-treated mutation in maintainer rice ‘Xinong 1B’. The mutant sterile lemma elongation and widen, serious silicide surface cells, with a large number of burr which like the burr on lemma, and the morphological structure dissection of sterile lemma is similar to the lemma. The results showed that the linkage relation of LSL locus was located between markers RM1085 and RM6663 on the short arm of chromosome 7, the physical distance is 735.5 kb. This result provided a foundation of map-based cloning and function analysis of LSL gene.Key words: rice; spikelet; sterile lemma; lsl (lemma-like sterile lemma); genetic analysis 全文下载PDF版本此摘要已有 2688 人浏览 |
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