基于黑磷

纳米酶介导的 3,3',5,5'-四甲基联苯胺 (TMB) - H 2 O 2系统催生了多种比色传感平台的建立，这些平台是有效的，但有时灵敏度较低。以温度为输出信号，光热效应为灵敏检测带来了新的策略。在本文中，构建了一种用于己烯雌酚 (DES) 的比色和光热双模式免疫测定。它基于黑磷-金纳米颗粒（BP/Au）纳米杂化物催化TMB的氧化反应，首次详细研究了纳米杂化物的动力学和催化机理。在此，纳米杂化物起到催化和光热双重作用。此外，TMB（氧化TMB）的单电子氧化产物不仅可以作为显色剂，而且还是一种优良的近红外激光驱动光热剂。温度 (ΔT/°C) 由便携式数字温度计测量。通过间接竞争策略，提出了一种简单、灵敏且经济的免疫传感器。样品中较高的 DES 含量与较少的 BP/Au 纳米杂化物结合到 ELISA 微孔板表面、较弱的颜色变化和暴露于激光照射时较低的温度变化相关。该方法用于实际样品中DES的测定，回收率令人满意，在食品安全检测应用中显示出广阔的前景。暴露于激光照射时温度变化较小。该方法用于实际样品中DES的测定，回收率令人满意，在食品安全检测应用中显示出广阔的前景。暴露于激光照射时温度变化较小。该方法用于实际样品中DES的测定，回收率令人满意，在食品安全检测应用中显示出广阔的前景。

"点击查看英文标题和摘要"

Nanozyme-mediated 3,3′,5,5′-tetramethylbenzidine (TMB) - H2O2 systems have spawned the establishment of multiple colorimetric sensing platforms that are effective but sometimes subject to low sensitivity. Taking temperature as the output signal, photothermal effects lead to new strategies for sensitive detection. In this paper, a colorimetric and photothermal dual-mode immunoassay for diethylstilbestrol (DES) was constructed. It is based on the oxidation reaction of TMB catalyzed by black phosphorus-gold nanoparticle (BP/Au) nanohybrids, and the kinetics as well as catalytic mechanism of the nanohybrids were investigated in detail for the first time. Herein, the nanohybrids playcatalytic and photothermal dual roles. Moreover, the one-electron oxidation product of TMB (oxidized TMB) not only acts as chromogenic agent but also an excellent NIR laser-driven photothermal agent. The temperature (ΔT/°C) was gauged by a portable digital thermometer. Through an indirect competition strategy, a simple, sensitive, and economic immunosensor was proposed. Higher DES content in the sample correlated with less BP/Au nanohybrids conjugated to the surface of ELISA microplate, a weaker color change, and a lower temperature variation when exposed to laser irradiation. This method was applied for DES determination in real samples with gratifying recovery rates, showing great promise in food safety inspection applications.