限制性酶切位点表

2024-03-06 06:00| 来源: 网络整理| 查看: 265

限制性酶切位点表广告

限制酶，也称为限制性核酸内切酶，是一组切割 DNA 的酶，天然存在于细菌中。它们帮助细菌抵抗病毒感染（即噬菌体）。限制酶被认为是自然界的分子剪刀，因为它们在 DNA 中寻找特定的核苷酸序列，称为“限制位点”，它们可以结合这些序列，然后通过水解磷酸二酯主链将其剪成碎片。限制性片段可以有平端，有 5'-磷酸基团帮助它们粘在一起，也可以有粘性末端，有 3'- 或 5'- 悬垂的 XNUMX 到 XNUMX 个核苷酸并且更稳定。这些短的单链 DNA 片段可以连接到自身或连接到具有匹配区域的另一段 DNA。由于片段化以可预测的方式发生在识别位点或附近，因此限制酶已成为基因工程（例如 DNA 克隆和 DNA 指纹识别）中的重要工具。

不同种类的限制酶

不同类型的细菌会产生限制酶，切割不同的核苷酸序列。科学家们已经发现并清理了数百种限制性内切酶，并根据它们的构成、切割位点的外观以及酶作为辅助因子的需要将它们分为四组。

I 型限制酶在距限制位点约 1000 bp 的随机位置切割。作为酶的辅助因子，您需要 ATP、AdoMet 和 Mg2+。 EcoK I、EcoA I 和 CfrA I 都是 I 型限制酶的例子。II 型限制酶在限制位点附近或恰好切割，它们需要 Mg2+ 作为酶辅因子。 EcoR I、BamH I 和 Hind III 都是 II 型限制酶的例子。III 型限制酶在距限制位点 24 至 26 bp 的位点切割。需要 ATP 和 Mg2+ 作为这些酶的辅助因子。 EcoP I、EcoP15 I 和 Hinf III 都是 II 型限制酶的例子。IV 型限制性内切酶在限制性位点附近或短距离处切割。它们还靶向修饰的 DNA，例如甲基化、羟甲基化和葡糖基羟甲基化 DNA。A = 腺嘌呤C = 胞嘧啶G = 鸟嘌呤T = 胸腺嘧啶N = A、C、G 或 TM = A 或 CR = A 或 GW = A 或 TY = C 或 TS = C 或 GK = G 或 TH = A、C 或 TB = C、G 或 TV = A、C 或 GD = A、G 或 T酶识别序列切悬垂AATⅡ5'- GACGT/C -'33'- C/TGCAG -'55'-GACGT C-3'3′-C TGCAG-5′3' ACGT阿巴斯5'- CNNNNNNNNNNN/NNNNNNNNNG -'33'- GNNNNNNNNN/NNNNNNNNNNNC -'55'-CNNNNNNNNNNNN NNNNNNNNNG-3'3′-GNNNNNNNNN NNNNNNNNNNNC-5′3′神经网络ACC65I5'- G/GTACC -'33'- CCATG/G -'55′-G GTACC-3′3′-CCATG G-5′5' GTAC加速器5'- GT/MKAC -'33'- CAKM/TG -'55′-GT MKAC-3′3′-CAKM TG-5′5' 米高酸碱度5'- C/CGC -'33'- GGC/G-'55′-C CGC-3′3′-GGC G-5′5' 电脑图ACLI5'- AA/CGTT -'33'- TTGC/AA -'55′-AA CGTT-3′3′-TTGC AA-5′5' 电脑图亚库尔5'-CTGAAGN14 NN/-'33′-GACTTCN14/NN-'55′-CTGAAGN14神经网络 -3′3′-GACTTCN14 NN-5′3′神经网络艾菲尔5'- AGC/GCT -'33'- TCG/CGA -'55′-AGC GCT-3′3′-TCG CGA-5′钝艾菲尔5'- C/TTAAG -'33′-GAATT/C-'55′-C TTAAG-3′3′-GAATT C-5′5′TTAA爱丽丝5'- A/CRYGT-'33'- TGYRC/A -'55'-A CRYGT-3'3′-TGYRC A-5′5' CRYG年龄I5'- A/CCGGT-'33'- TGGCC/A -'55'-A CCGGT-3'3′-TGGCC A-5′5'CCGG阿赫迪5'- GACNNN/NNGTC -'33'- CTGNN/NNNCAG -'55′-GACNNN NNGTC-3′3′-CTGNN NNNCAG-5′3′ 正AleI-v25'- CACNN/NNGTG -'33'- GTGNN/NNCAC -'55′-CACNN NNGTG-3′3′-GTGNN NNCAC-5′钝铝5'- AG/CT -'33'- TC/GA -'55′-AG CT-3′3′-TC GA-5′钝阿尔维5′- GGATCN4/N-'33'- CCTAGN4 N/-'55′-GGATCN4 N-3′3′-CCTAGN4N -5′5′ 正阿尔维尼5'- CAGNNN/CTG -'33'- GTC/NNNGAC -'55′-CAGNNN CTG-3′3′-GTC NNNGAC-5′3′NNN阿帕尔5'- GGGCC/C -'33'- C/CCGGG -'55′-GGGCC C-3′3′-C CCGGG-5′3' GGCC阿帕里5'- G/TGCAC -'33′- CACGT/G-'55′-G TGCAC-3′3′-CACGT G-5′5' TGCA载脂蛋白I5'- R/AATTY -'33'- YTTAA/R -'55'-R AATTY-3'3′-YTTAA R-5′5′AATT升序码5'- GG/CGCGCC -'33'- CCGCGC/GG -'55′-GG CGCGCC-3′3′-CCGCGC GG-5′5' CGCG阿塞尔5'- AT/TAAT -'33'- TAAT/TA -'55'-AT TAAT-3'3′-TAAT TA-5′5'助教阿西西5'- GCGAT/CGC -'33'- CGC/TAGCG -'55′-GCGAT CGC-3′3′-CGC TAGCG-5′3' 在有用的BsoBI5'- C/YCGRG -'33'- GRGCY/C-'55'-C YCGRG-3'3′-GRGCY C-5′5' YCGR阿凡达5'- G/GWCC -'33'- CCWG/G -'55′-G GWCC-3′3'-CCWG G-5'5' GWC艾薇儿5'- C/CTAGG-'33'- GGATC/C -'55'-C CTAGG-3'3′-GGATC C-5′5' CTAG贝吉5'- GKGCM/C -'33'- C/MCGKG -'55′-GKGCM C-3′3'-C MCGKG-5'3′KGCM贝尔5′- NNNNN/N10 ACNNNNGTAYCN7 NNNNN/-'33′-/NNNNNN10 TGNNNNCATRGN7/NNNNN-'55′-NNNNN N10阿CNNNGTAYCN7NNNNN -3′3'- NNNNNN10TGNNNNCATTRGN7 NNNNN-5′3′ NNNNN巴姆HI5'- G/GATCC -'33'- CCTAG/G-'55′-G GATCC-3′3′-CCTAG G-5′5' 关贸总协定班尼5'- G/GYRCC -'33'- CCRYG/G-'55′-G GYRCC-3′3′-CCRYG G-5′5' GYRC班II5'- GRGCY/C-'33'- C/YCGRG -'55′-GRGCY C-3′3'-C YCGRG-5'3' RGCYBBSI5′-GAAGACN2/NNNN-'33′-CTTCTGN2 NNNN/-'55′-GAAGACN2 NNNN-3′3′-CTTCTGGN2NNNN -5′5′ NNNN胆汁酸5'- CC/TCAGC -'33'- GGAGT/CG -'55′-CC TCAGC-3′3′-GGAGT CG-5′5' 三氯乙酸巴维5′-GCAGCN8/NNNN-'33′- CGTCGN8 NNNN/-'55′-GCAGCN8 NNNN-3′3′-CGTCGN8NNNN -5′5′ NNNNBCCI5′-CCATCN4/N-'33′- GGTAGN4 N/-'55′-CCATCN4 N-3′3′-GGTAGN4N -5′5′ 正人工智能5′-ACGGCN12/NN-'33′-TGCCGN12 NN/-'55′-ACGGCN12 NN-3′3′-TGCCGN12神经网络 -5′5′神经网络BCGI5′- NN/N10 CGANNNNNNTGCN10 NN/-'33′-/NNN10 GCTNNNNNNNNACGN10/NN-'55′-NN N10CGANNNNNNTGCN10神经网络 -3′3′- NNN10GCTNNNNNNNNACGN10 NN-5′3′神经网络BciVI5′-GTATCCN5 N/-'33'- CATAGGN5/N-'55′-GTATCCN5N -3′3′-CATAGN5 N-5′3′ 正BclI5'- T/GATCA -'33'- ACTAG/T -'55'-T GATCA-3'3′-ACTAG T-5′5' 关贸总协定巴法尔5'- C/TAG -'33'-盖特/C-'55'-C TAG-3'3′-GAT C-5′5'助教BglI5'- GCCNNNN/NGGC -'33'- CGGN/NNNNCCG -'55′-GCCNNNN NGGC-3′3′-CGGN NNNNCCG-5′3′NNNBglⅡ5'- A/关贸总协定 -'33'- TCTAG/A -'55'-A GATCT-3'3′-TCTAG A-5′5' 关贸总协定BPI5'- GC/TNAGC -'33'- CGANT/CG -'55′-GC TNAGC-3′3′-CGANT CG-5′5' TNA溴化酶B5'- CAC/GTC -'33'- GTG/CAG -'55′-CAC GTC-3′3′-GTG CAG-5′钝溴化物5'- ACTGGGN4 N/-'33′-TGACCCN4/N-'55′-ACTGGGN4N -3′3′-TGACCCN4 N-5′3′ 正骨髓基质蛋白I5'- GCTAG/C -'33'- C/GATCG -'55′-GCTAG C-3′3′-C GATCG-5′3' CTAG心率指数5′-CTGGAGN14 NN/-'33′-广域核糖核酸14/NN-'55′-CTGGAGGN14神经网络 -3′3′-GACCTCN14 NN-5′3′神经网络BPU10I5'- CC/TNAGC -'33'- GGANT/CG -'55′-CC TNAGC-3′3′-GGANT CG-5′5' TNABPUEI5'-CTTGAGN14 NN/-'33′-GAACTCN14/NN-'55′-CTTGAGN14神经网络 -3′3′-GAACTCN14 NN-5′3′神经网络牛血清蛋白AI5'- YAC/GTR -'33'- RTG/CAY -'55′-YAC GTR-3′3′-RTG CAY-5′钝血清BsaBI5'- GATNN/NNATC -'33'- CTANN/NNTAG -'55′-GATNN NNATC-3′3′-CTANN NNTAG-5′钝牛血清白蛋白5'- GR/CGYC -'33'- CYGC/RG -'55′-GR CGYC-3′3′-CYGC RG-5′5' 电脑图BSAL5'- GGTCTCN/NNNN -'33'- CCAGAGNNNNN/-'55′-GGTCTCN NNNN-3′3′-CCAGAGNNNNN -5′5′ NNNN巴沙吉5'- C/CNNG-'33'- GGNNC/C -'55′-C CNNGG-3′3′-GGNNC C-5′5′中国天然气集团公司血清白蛋白5'- W/CCGGW -'33'- WGGCC/W -'55'-W CCGGW-3'3′-WGGCC W-5′5'CCGGBsaXI5′- NNN/N9 ACNNNNNCTCCN7 NNN/-'33′-/NNNN9 TGNNNNGAGGN7/NNN-'55′-NNN N9ACNNNNNCTCCN7NNN -3′3′- NNNN9TGNNNNGAGGN7 NNN-5′3′NNNBSERI5'- 恶作剧8 NN/-'33′-CTCCTCN8/NN-'55'-GAGGAGN8神经网络 -3′3′-CTCCTCN8 NN-5′3′神经网络BseYI5'- C/CCAGC-'33'- GGGTC/G -'55'-C CCAGC-3'3′-GGGTC G-5′5'CCAG乙硫氨酸5'- GTGCAGN14 NN/-'33′- CACGTCN14/NN-'55′-GTGCAGN14神经网络 -3′3′-CACGTCN14 NN-5′3′神经网络BSIEI5'- CGRY/CG -'33'- GC/YRGC -'55′-CGRY CG-3′3′-GC YRGC-5′3' RYBsiHKAI5'- GWGCW/C -'33'- C/WCGWG -'55'-GWGCW C-3'3'-C WCGWG-5'3' WGCWBSIWI5'- C/GTACG -'33'- GCATG/C -'55′-C GTACG-3′3′-GCATG C-5′5' GTACBSII5'- CCNNNNNN/NNGG -'33'- GGNN/NNNNNCC -'55′-CCNNNNNN NNGG-3′3′-GGNN NNNNNCC-5′3′NNNBsmAI BcoDI5'- GTCTCN/NNNN -'33'- CAGAGNNNNN/-'55′-GTCTCN NNNN-3′3′-CAGAGNNNNN -5′5′ NNNNBsmBI-v25'- CGTCTCN/NNNN -'33'- GCAGAGNNNNN/-'55′-CGTCTCN NNNN-3′3′-GCAGAGNNNNN -5′5′ NNNNBSMFI5'- GGGACN10/NNNN-'33′-CCCTGN10 NNNN/-'55′-GGGACN10 NNNN-3′3′-CCCTGN10NNNN -5′5′ NNNN贝斯米5'- GAATGCN/-'33′-CTTAC/GN-'55′-GAATGCN -3′3′-CTTAC GN-5′3' CNBSP1286I5'- GDGCH/C -'33'- C/HCGDG -'55′-GDGCH C-3′3′-C HCGDG-5′3' DGCHBspCNI5′-CTCAGN7 NN/-'33′-GAGTCN7/NN-'55′-CTCAGN7神经网络 -3′3′-GAGTCN7 NN-5′3′神经网络酶联免疫吸附试验5'- T/CCGGA -'33'- AGGCC/T -'55'-T CCGGA-3'3′-AGGCC T-5′5'CCGGBSPHI5'- T/CATGA -'33'- AGTAC/T -'55′-T CATGA-3′3′-AGTAC T-5′5' CATGBspMI BfuAI5′-ACCTGCN4/NNNN-'33′-TGGACGN4 NNNN/-'55′-ACCTGCN4 NNNN-3′3′-TGGACGN4NNNN -5′5′ NNNN溴化钾5'- CCG/CTC -'33'- GGC/GAG -'55′-CCG CTC-3′3′-GGC GAG-5′钝溴化硅5'- GCAATGNN/-'33'- CGTTAC/NN -'55′-GCAATGNN -3′3′-CGTTAC NN-5′3′神经网络BsrFI-v25'- R/CCGGY -'33'- YGGCC/R -'55′-R CCGGY-3′3′-YGGCC R-5′5'CCGGBSRGI5'- T/侠盗猎车手 -'33'- ACATG/T -'55'-T GTACA-3'3′-ACATG T-5′5' GTACBSRI5'- ACTGGN/-'33'- TGAC/CN -'55'-ACTGGN -3'3′-TGAC CN-5′3′肾上腺素BSSHII5'- G/CGCGC -'33'- CGCGC/G -'55′-G CGCGC-3′3′-CGCGC G-5′5' CGCGBSSSI-v25'- C/ACGAG -'33'- GTGCT/C -'55'-C ACGAG-3'3′-GTGCT C-5′5' ACGABstAPI5'- GCANNNN/NTGC -'33'- CGTN/NNNNACG -'55′-GCANNNN NTGC-3′3′-CGTN NNNNACG-5′3′NNNBstBI5'- TT/CGAA -'33'- AAGC/TT -'55′-TT CGAA-3′3′-AAGC TT-5′5' 电脑图BstEII BstEII5'- G/GTNACC -'33'- CCANTG/G -'55′-G GTNACC-3′3′-CCANTG G-5′5' GTNACBstNI5'- CC/WGG -'33'- GGW/CC -'55'-CC WGG-3'3′-GGW CC-5′5' 宽BstUI5'- CG/CG -'33'-气相色谱/气相色谱-'55′-CG CG-3′3′-GC GC-5′钝BstXI5'- CCANNNNN/NTGG -'33'- GGTN/NNNNNACC -'55′-CCANNNNN NTGG-3′3′-GGTN NNNNNACC-5′3′ NNNNBstYI5'- R/GATCY -'33′-YCTAG/R-'55'-R GATCY-3'3′-YCTAG R-5′5' 关贸总协定BstZ17I5'- GTA/TAC -'33'- CAT/ATG -'55′-GTA TAC-3′3′-CAT ATG-5′钝Bsu36I5'- CC/TNAGG -'33'- GGANT/CC -'55′-CC TNAGG-3′3′-GGANT CC-5′5' TNABgI5'- C/CRYGG -'33'- GGYRC/C -'55'-C CRYGG-3'3′-GGYRC C-5′5' CRYGBgZI5′-GCGATGN10/NNNN-'33′- CGCTACN10 NNNN/-'55′-GCGATGN10 NNNN-3′3′-CGCTACN10NNNN -5′5′ NNNNBTSCI5'- GGATGNN/-'33'- CCTAC/NN -'55′-GGATGNN -3′3′-CCTAC NN-5′3′神经网络BtsI-v25'- GCAGTGNN/-'33'- CGTCAC/NN -'55′-GCAGTGNN -3′3′-CGTCAC NN-5′3′神经网络BTSIMutI5'- CAGTGNN/-'33'- GTCAC/NN -'55'-CAGTGNN -3'3′-GTCAC NN-5′3′神经网络Cac8I5'- GCN/NGC -'33'- CGN/NCG -'55′-GCN NGC-3′3′-中广核NCG-5′钝类BspDI5'- AT/CGAT -'33'- TAGC/TA -'55′-AT CGAT-3′3′-TAGC TA-5′5' 电脑图铯CspCI5′- NN/N11 CAANNNNNTGGN10 NN/-'33′-/NNN11 GTTNNNNNCACCN10/NN-'55′-NN N11CAANNNNNTGGN10神经网络 -3′3′- NNN11GTTTNNNNNCACCCN10 NN-5′3′神经网络CviAII5′- C/ATG-'33'- GTA/C -'55'-C ATG-3'3′-GTA C-5′5' 在CviKI-15'- RG/CY -'33'- YC/GR -'55'-RG CY-3'3′-YC GR-5′钝质量指数5'- G/TAC -'33′- CAT/G-'55'-G TAC-3'3′-CAT G-5′5'助教删除5'- C/TNAG -'33'-甘特/C-'55′-C TNAG-3′3′-GANT C-5′5' TNA压差5′-GA/TC-'33'- CT/AG -'55′-GA TC-3′3′-CT AG-5′钝德拉尔5'- TTT/AAA -'33'- AAA/TTT -'55′-TTT AAA-3′3′-AAA TTT-5′钝德拉III5'- CACNNN/GTG -'33'- GTG/NNNCAC -'55′-CACNNN GTG-3′3′-GTG NNNCAC-5′3′NNNdrdI5'- GACNNNN/NNGTC -'33'- CTGNN/NNNNCAG -'55′-GACNNNN NNGTC-3′3′-CTGNN NNNNCAG-5′3′神经网络EaI5′-Y/GGCCR-'33'- RCCGG/Y -'55′-Y GGCCR-3′3′-RCCGG Y-5′5' GGCC伊格尔5'- C/GGCCG -'33'- GCCGG/C -'55′-C GGCCG-3′3′-GCCGG C-5′5' GGCC耳朵5'-CTCTTCN/NNN-'33'- GAGAAGNNNN/-'55′-CTCTTTCN NNN-3′3′-GAGAAGNNNN -5′5′NNN电子书5'- GGCGGAN9 NN/-'33′-CCGCCTN9/NN-'55'-GGCGGAN9神经网络 -3′3′-CCGCCTN9 NN-5′3′神经网络生态53kI5'- GAG/CTC -'33'- CTC/GAG -'55′-GAG CTC-3′3′-CTC GAG-5′钝经济NI5'- CCTNN/NNNAGG -'33'- GGANNN/NNTCC -'55′-CCTNN NNNAGG-3′3′-GGANNN NNTCC-5′5′ 正生态O109I5'- RG/GNCCY -'33'- YCCNG/GR -'55'-RG GNCCY-3'3′-YCCNG GR-5′5′健安喜生态P15I5′-CAGCAGN25/NN-'33′- GTCGTCN25 NN/-'55′-CAGCAGN25 NN-3′3′-GTCGTCN25神经网络 -5′5′神经网络生态研究所5'- G/AATTC -'33′-CTTAA/G-'55′-G AATTC-3′3′-CTTAA G-5′5′AATT生态房车5′-盖特/ATC-'33'- CTA/标签 -'55′-GAT ATC-3′3′-CTA TAG-5′钝埃斯普3I5'- CGTCTCN/NNNN -'33'- GCAGAGNNNNN/-'55′-CGTCTCN NNNN-3′3′-GCAGAGNNNNN -5′5′ NNNN脂肪5′-/CATG-'33'- GTAC/-'55'- CATG-3'3′-GTAC -5′5' CATG故障5'- CCCGCN4/NN-'33'- GGGCGN4 NN/-'55′-CCCGCN4 NN-3′3′-GGGCGN4神经网络 -5′5′神经网络Fnu4HI5'- GC/NGC -'33'- CGN/CG -'55′-GC NGC-3′3′-CGN CG-5′5′ 正福克5'- GGATGN9/NNNN-'33′- 碳化氮9 NNNN/-'55′-GGATGN9 NNNN-3′3′-CCTACN9NNNN -5′5′ NNNN荧光素酶I5'- GGCCGG/CC -'33'- CC/GGCCGG -'55′-GGCCGG CC-3′3′-CC GGCCGG-5′3'CCGGFSPEI5'-CCN12/NNNN-'33′- GGN12 NNNN/-'55′-CCN12 NNNN-3′3′-GGN12NNNN -5′5′ NNNNFspI5'- TGC/GCA -'33'- ACG/CGT -'55′-TGC GCA-3′3′-ACG CGT-5′钝海尔5'- RGCGC/Y -'33'- Y/CGCGR -'55′-RGCGC Y-3′3′-Y CGCGR-5′3' GCGC海三5'- GG/CC -'33′-CC/GG-'55′-GG CC-3′3′-CC GG-5′钝汞5′-广汽GCN5/NNNNN-'33′-CTGCGN5 NNNNN/-'55′-GACGCN5 NNNNN-3′3′-CTGCGN5NNNNN -5′5′ NNNNN哈尔5'- GCG/C -'33'- C/GCG -'55′-GCG C-3′3′-C GCG-5′3' 电脑图欣P1I5'- G/CGC -'33'- CGC/G-'55′-G CGC-3′3′-CGC G-5′5' 电脑图欣奇5'- GTY/RAC -'33'- 汽车/YTG -'55′-GTY RAC-3′3′-CAR YTG-5′钝后III5'- A/AGCTT -'33'- TTCGA/A -'55'-A AGCTT-3'3′-TTCGA A-5′5′AGCT欣菲5'- G/ANTC -'33'-CTNA/G-'55'-G ANTC-3'3′-CTNA G-5′5'蚂蚁帕伊尔5'- GTT/AAC -'33'- CAA/TTG -'55′-GTT AAC-3′3′-CAA TTG-5′钝HphI5'- GGTGAN7 N/-'33′- CCACTN7/N-'55'-GGTGAN7N -3′3′-CCACTN7 N-5′3′ 正Hpy166II5'- GTN/NAC -'33'- CAN/NTG -'55′-GTN NAC-3′3′-CAN NTG-5′钝Hpy188I5'- TCN/GA -'33'- AG/NCT -'55'-TCN GA-3'3′-AG NCT-5′3′ 正Hpy188III5'- TC/NNGA -'33'- AGNN/CT -'55′-TC NNGA-3′3′-AGNN CT-5′5′神经网络Hpy99I5'- CGWCG/-'33′-/GCWGC-'55′-CGWCG -3′3'- GCWGC-5'3' CGWCGHpyAV5′- CCTTCN5 N/-'33'- GGAAGN5/N-'55′-CCTTCN5N -3′3′-GGAAGN5 N-5′3′ 正HpyCH4III5'-乙腈/GT-'33'- TG/NCA -'55'-ACN GT-3'3′-TG NCA-5′3′ 正HpyCH4IV5'- A/CGT -'33'- TGC/A -'55'-A CGT-3'3′-TGC A-5′5' 电脑图HpyCH4V5'- TG/CA -'33'- AC/GT -'55'-TG CA-3'3′-AC GT-5′钝细胞5'- TAACTATAACGGTCCTAA/GGTAGCGAA -'33'- ATTGATATTGCCAG/GATTCCATCGCTT -'55'-TAACTATAACGGTCCTAA GGTAGCGAA-3'3′-ATTGATATTGCCAG GATTCCATCGCTT-5′3' CTAAI-SceI5'- TAGGGATAA/CAGGGTAAT -'33'- ATCCC/TATTGTCCCATTA -'55'-TAGGGATAA CAGGGTAAT-3'3'-ATCCC TATTGTCCCCATTA-5'3′ATAA卡西5'- G/GCGCC -'33'- CCGCG/G -'55′-G GCGCC-3′3′-CCGCG G-5′5' GCGCKpnI5'- GGTAC/C -'33'- C/CATGG -'55′-GGTAC C-3′3′-C CATGG-5′3' GTAC脂蛋白5′- CCDGN10/NNNN-'33′- GGHCN10 NNNN/-'55′-CCDGN10 NNNN-3′3′-GGHCN10NNNN -5′5′ NNNNMboI Sau3AI DpnII5′-/GATC-'33'-CTAG/-'55'- GATC-3'3′-CTAG -5′5' 关贸总协定MboⅡ5'- 加根7 N/-'33′-CTTCTN7/N-'55′-加根7N -3′3′-CTTCTN7 N-5′3′ 正生产率5'- C/AATTG -'33'- GTTAA/C -'55'-C AATTG-3'3′-GTTAA C-5′5′AATT氯化铝5′-/AATT-'33'- TTAA/-'55'- AATT-3'3′-TTAA -5′5′AATT姆卢尔5'- A/CGCGT -'33'- TGCGC/A -'55'-A CGCGT-3'3′-TGCGC A-5′5' CGCG米利尔5′-GAGTCN5/-'33′-CTCAGN5/-'55′-GAGTCN5 -3'3′-CTCAGN5 -5'钝梅尔5'- TCCRACN18 NN/-'33'- AGGYTGN18/NN-'55′-TCCRACN18神经网络 -3′3′-AGGYTGN18 NN-5′3′神经网络锰矿5′-中控通6 N/-'33'- GGAGN6/N-'55′-CCTCN6N -3′3′-GGAGN6 N-5′3′ 正理学硕士5'- TGG/CCA -'33'- ACC/GGT -'55′-TGG CCA-3′3′-ACC GGT-5′钝MSEI5'- T/TAA -'33'- AAT/T -'55'-T TAA-3'3′-AAT T-5′5'助教微信号5'- CAYNN/NNRTG -'33'- GTRNN/NNYAC -'55′-CAYNN NNRTG-3′3′-GTRNN NNYAC-5′钝MspA1I5'- CMG/CKG -'33'- GKC/GMC -'55′-CMG CKG-3′3′-GKC GMC-5′钝MspI HpaII5'- C/CGG -'33'- GGC/C -'55'-C CGG-3'3′-GGC C-5′5' 电脑图MspJI5′-CNNRN9/NNNN-'33'- GNNYN9 NNNN/-'55′-CNNRN9 NNNN-3′3′-GNNYN9NNNN -5′5′ NNNN最小值5'- GCNNNNNN/NNGC -'33'- CGNN/NNNNNCG -'55′-GCNNNNNN NNGC-3′3′-CGNN NNNNNCG-5′3′NNN奈尔5'- GCC/GGC -'33'- CGG/CCG -'55′-GCC GGC-3′3′-CGG CCG-5′钝纳尔5'- GG/CGCC -'33'- CCGC/GG -'55′-GG CGCC-3′3′-CCGC GG-5′5' 电脑图NCII5'- CC/SGG -'33'- GGS/CC -'55′-CC SGG-3′3′-GGS CC-5′5' 秒NCOI5'- C/CATGG -'33'- GGTAC/C -'55′-C CATGG-3′3′-GGTAC C-5′5' CATG钕铁硼5'-CA/TATG-'33′-GTAT/AC-'55′-CA TATG-3′3′-GTAT AC-5′5'助教恩戈米病毒5'- G/CCGGC -'33′- CGGCC/G-'55′-G CCGGC-3′3′-CGGCC G-5′5'CCGG尼尔5'- G/CTAGC -'33′- CGATC/G-'55′-G CTAGC-3′3′-CGATC G-5′5' CTAGNlaⅢ5'- CATG/-'33′-/GTAC-'55′-CATG -3′3'- GTAC-5'3' CATGNlaIV5'- GGN/NCC -'33'- CCN/NGG -'55′-GGN NCC-3′3′-CCN NGG-5′钝NmeAIII5'- GCCGAGN19 NN/-'33′- CGGCTCN19/NN-'55′-GCCGAGN19神经网络 -3′3′-CGGCTCN19 NN-5′3′神经网络不是我5'- GC/GGCCGC -'33'- CGCCGG/CG -'55′-GC GGCCGC-3′3′-CGCCGG CG-5′5' GGCCru5'- TCG/CGA -'33'- AGC/GCT -'55′-TCG CGA-3′3′-AGC GCT-5′钝尼西尔5'- ATGCA/T -'33'- T/ACGTA -'55′-ATGCA T-3′3′-T ACGTA-5′3' TGCA内切酶I5'- RCATG/Y -'33′- Y/GTACR-'55′-RCATG Y-3′3′-Y GTACR-5′3' CATGNt.AlwI5'- GGATCNNNN/-'33′-/CCTAGNNNN-'55′-GGATCNNNN -3′3'- CCTAGNNNN-5'3′ GGATCNNNNNt.BbvCI5'- CC/TCAGC -'33′-/GGAGTCG-'55′-CC TCAGC-3′3'- GGAGTCG-5'3'抄送Nt.BsmAI5'- GTCTCN/-'33′-/CAGAGN-'55′-GTCTCN -3′3'- CAGAGN-5'3' GTCTCNNt BspQI5'- GCTCTTCN/-'33′-/CGAGAAGN-'55′-GCTCTTCN -3′3'- CGAGAGN-5'3' GCTCTTCNNt.BstNBI5'- GAGTCNNNN/-'33′-/CTCAGNNNN-'55′-GAGTCNNNN -3′3'-CTCAGNNNN-5'3' GAGTCNNNNPI-PspI5'- TGGCAAACAGCTATTAT/GGGTATTATGGGT -'33'- ACCGTTTGTCGAT/AATACCCATAATACCCA -'55'-TGGCAAACAGCTATTAT GGGTATTATGGGT-3'3′-ACCGTTTGTCGAT AATACCCATAATACCCA-5′3′ TTATPI-场景5'- ATCTATGTCGGGTGC/GGAGAAAGAGGTAAT -'33'- TAGATACAGCC/CACGCCTCTTTTCTCCATA -'55'-ATCTATGTCGGGTGC GGAGAAAGAGGTAAT-3'3'-TAGATACAGCC CACGCCTCTTTTCTCCATA-5'3' GTGC聚酰亚胺5'- TTAAT/TAA -'33'- AAT/TAATT -'55′-TTAAT TAA-3′3′-AAT TAATT-5′3' 在PCII5'- A/CATGT -'33'- TGTAC/A -'55'-A CATGT-3'3′-TGTAC A-5′5' CATGPMI5'- CCANNNN/NTGG -'33'- GGTN/NNNNACC -'55′-CCANNNN NTGG-3′3′-GGTN NNNNACC-5′3′NNN普尔5′-GAGTCN4/N-'33′-CTCAGN4 N/-'55′-GAGTCN4 N-3′3′-CTCAGN4N -5′5′ 正普鲁蒂5'- GGCGC/C -'33'- C/CGCGG -'55′-GGCGC C-3′3′-C CGCGG-5′3' GCGC普梅尔5'- GTTT/AAAC -'33'- CAAA/TTTG -'55′-GTTT AAAC-3′3′-CAAA TTTG-5′钝颗粒物5'- CAC/GTG -'33'- GTG/CAC -'55′-CAC GTG-3′3′-GTG CAC-5′钝PpuMI5'- RG/GWCCY -'33'- YCCWG/GR -'55'-RG GWCCY-3'3'-YCCWG GR-5'5' GWC人工智能5'- GACNN/NNGTC -'33'- CTGNN/NNCAG -'55′-GACNN NNGTC-3′3′-CTGNN NNCAG-5′钝PsiII-v25'- TTA/TAA -'33'- AAT/ATT -'55′-TTA TAA-3′3′-AAT ATT-5′钝PspGI5'-/CCWGG-'33'- GGWCC/-'55'- CCWGG-3'3′-GGWCC -5′5' CCWGGPspOMI5'- G/GGCCC -'33'- CCCGG/G-'55′-G GGCCC-3′3′-CCCGG G-5′5' GGCCPspXI5'- VC/TCGAGB -'33'- BGAGCT/CV -'55′-VC TCGAGB-3′3′-BGAGCT CV-5′5′TCGA聚苯乙烯5'- CTGCA/G -'33'- G/ACGTC -'55′-CTGCA G-3′3′-G ACGTC-5′3' TGCAPuI5'- CGAT/CG -'33'- GC/TAGC -'55′-CGAT CG-3′3′-GC TAGC-5′3' 在PvII5'- CAG/CTG -'33'- GTC/GAC -'55′-CAG CTG-3′3′-GTC GAC-5′钝拉萨尔5'- GT/AC -'33′-CA/TG-'55′-GT AC-3′3′-CA TG-5′钝抗逆转录酶II5'- CG/GWCCG -'33'- GCCWG/GC -'55′-CG GWCCG-3′3'-GCCWG GC-5'5' GWC萨克尔5′-GAGCT/C-'33'- C/TCGAG -'55′-GAGCT C-3′3′-C TCGAG-5′3′AGCT萨克尔5'- CCGC/GG -'33'- GG/CGCC -'55′-CCGC GG-3′3′-GG CGCC-5′3′气相色谱萨尔5'- G/TCGAC -'33′-CAGCT/G-'55′-G TCGAC-3′3′-CAGCT G-5′5′TCGASAPI BspQI5'- GCTCTTCN/NNN -'33'- CGAGAAGNNNN/-'55′-GCTCTTCN NNN-3′3′-CGAGAAGNNNN -5′5′NNN绍96I5'- G/GNCC -'33'- CCNG/G -'55′-G GNCC-3′3′-CCNG G-5′5′健安喜碘化锑5'- CCTGCA/GG -'33'- GG/ACGTCC -'55′-CCTGCA GG-3′3′-GG ACGTCC-5′3' TGCA量表5'- AGT/ACT -'33'- TCA/TGA -'55'-AGT ACT-3'3′-TCA TGA-5′钝荧光素5'- CC/NGG -'33'- GGN/CC -'55′-CC NGG-3′3′-GGN CC-5′5′ 正性AI5'- A/CCWGGT -'33'- TGGWCC/A -'55'-A CCWGGT-3'3′-TGGWCC A-5′5' CCWGG斯法尼5′-GCATCN5/NNNN-'33'- CGTAGN5 NNNN/-'55′-GCATCN5 NNNN-3′3′-CGTAGN5NNNN -5′5′ NNNN科学委员会5′- C/TRYAG-'33'- GAYRT/C -'55'-C TRYAG-3'3′-GAYRT C-5′5'特里亚SfI5'- GGCCNNNN/NGGCC -'33'- CCGGN/NNNNCCGG -'55′-GGCCNNNN NGGCC-3′3′-CCGGN NNNNCCGG-5′3′NNNSFOI5'- GGC/GCC -'33'- CCG/CGG -'55′-GGC GCC-3′3′-CCG CGG-5′钝狮群AI5'- CR/CCGGYG -'33'- GYGGCC/RC -'55′-CR CCGGYG-3′3′-GYGGCC RC-5′5'CCGG小号5'- CCC/GGG -'33'- GGG/CCC -'55′-CCC GGG-3′3′-GGG CCC-5′钝小号5'- C/蒂拉格 -'33'- 加里特/C -'55'-C TYRAG-3'3′-加里特 C-5′5'泰拉斯纳比5'- TAC/GTA -'33'- ATG/CAT -'55′-TAC GTA-3′3′-ATG CAT-5′钝斯佩尔5'- A/CTAGT-'33'- TGATC/A -'55'-A CTAGT-3'3′-TGATC A-5′5' CTAG螺旋体5'- GCATG/C -'33'- C/GTACG -'55′-GCATG C-3′3′-C GTACG-5′3' CATG六氟化硫5'- GCCC/GGGC -'33'- CGGG/CCCG -'55′-GCCC GGGC-3′3′-CGGG CCCG-5′钝SspI5'- AAT/ATT -'33'- TTA/TAA -'55′-AAT ATT-3′3′-TTA TAA-5′钝斯图尔5'- AGG/CCT -'33'- TCC/GGA -'55′-AGG CCT-3′3′-TCC GGA-5′钝斯泰D4I5′-/CCNGG-'33'- GGNCC/-'55'- CCNGG-3'3′-GGNCC -5′5' CCNGG手写笔5'- C/CWWGG -'33'- GGWWC/C -'55'-C CWWGG-3'3′-GGWWC C-5′5' CWWGSwal5'- ATTT/AAAT -'33'- TAAA/TTTA -'55'-ATTT AAAT-3'3′-TAAA TTTA-5′钝TaqI-v25'- T/CGA -'33'-自动增益控制/T-'55′-T CGA-3′3′-AGC T-5′5' 电脑图TfI5'- G/AWTC -'33'- CTWA/G -'55′-G AWTC-3′3′-CTWA G-5′5' AWT采尔猿5'- G/CWGC -'33'- CGWC/G -'55′-G CWGC-3′3′-CGWC G-5′5' CWGTsp45I5′-/GTSAC-'33'- CASTG/-'55'- GTSAC-3'3′-CASTG -5′5' GTSACTspRI5'- NNCASTGNN/-'33′-/NNGTSACNN-'55′-NNCASTGNN -3′3'- NNGTSACNN-5'3' NNCASTGNNTth111I PflFI5'- GACN/NNGTC -'33'- CTGNN/NCAG -'55′-GACN NNGTC-3′3′-CTGNN NCAG-5′5′ 正XbaI5'- T/CTAGA -'33'- AGATC/T -'55′-T CTAGA-3′3′-AGATC T-5′5' CTAGXcmI5'- CCANNNNN/NNNNTGG -'33'- GGTNNNN/NNNNNACC -'55′-CCANNNNN NNNNTGG-3′3′-GGTNNNN NNNNNACC-5′3′ 正XhoI PaeR7I5'- C/TCGAG -'33′-GAGCT/C-'55′-C TCGAG-3′3′-GAGCT C-5′5′TCGA圣诞节TspMI5'- C/CCGGG -'33'- GGGCC/C -'55′-C CCGGG-3′3′-GGGCC C-5′5'CCGGXmnI5'- GAANN/NNTTC -'33′-CTTNN/NNAAG-'55′-GAANN NNTTC-3′3′-CTTNN NNAAG-5′钝兹拉尔5'- GAC/GTC -'33'- CTG/CAG -'55′-GAC GTC-3′3′-CTG CAG-5′钝

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