重组Iba1抗体[EPR16588](ab178846)

Lane 1 : Human Iba1 recombinant protein at 0.1 µgLane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µgLane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µgLane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 30 µgLane 5 : Human spleen tissue lysate at 20 µgLane 6 : Mouse spleen tissue lysate at 30 µgLane 7 : Rat spleen tissue lysate at 30 µgLane 8 : U937 (human histiocytic lymphoma cell line) whole cell lysate at 30 µgLane 9 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µgLane 10 : THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µgLane 11 : THP-1 whole cell lysate, PMA treated at 30 µgLane 12 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µgLane 13 : C6 (rat glial tumor cell line) whole cell lysate at 30 µgLane 14 : NR8383 whole cell lysate at 30 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 17 kDa

Exposure time: 1 minute

Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA. 

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab178846 (anti-Iba1 antibody; 1/500 dilution) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG) at  1:50,000 dilution for 1 hour at room temperature and visualised using ECL development solution ab133406.