Phylogenetic Group, Biofilm Formation and Drug Resistance of Escherichia coli Isolated from Goose and Fish in Henan, China 您所在的位置:网站首页 animals/fish/s/9.jpg Phylogenetic Group, Biofilm Formation and Drug Resistance of Escherichia coli Isolated from Goose and Fish in Henan, China

Phylogenetic Group, Biofilm Formation and Drug Resistance of Escherichia coli Isolated from Goose and Fish in Henan, China

2023-04-22 01:17| 来源: 网络整理| 查看: 265

Background: Escherichia coli (E. coli) is a pathogen that sickens both humans and animals. There are relatively few reports on the correlation between drug resistance and biofilm formation ability of E.coli isolated from goose and fish in water environment of China. This study investigated the genetic typing, antibiotic sensitivity and biofilm formation ability of E. coli strains isolated from goose and fish.

Methods: The phylogenetic clustering, drug resistance and biofilm formation ability of E. coli were tested by triple PCR, crystal violet microplate and Microdilution method.聽

Result: The results showed the prevalence of group B2 and D in fish-derived E.coli accounted for 59% and 12%, goose-derived E. coli accounted for 48% and 9%, respectively. Some strains could form biofilm and biofilm formation ability was associated with the drug resistance. The MIC values of strains growing in biofilm were 2-16 times higher than those of corresponding planktonic bacteria. The antibiotic resistance among biofilm-forming isolates was significantly higher than that strains unable to form biofilm (p



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