HIV1 p24 ELISA试剂盒

HIV1 p24 ELISA Kit (ab218268) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HIV1 p24 protein in human serum, plasma, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate HIV1 p24 with 1.1 pg/mL sensitivity.

Lentivirus vectors based on HIV-1 have gained popularity as a tool for cell and gene therapy, owing to their ability to carry and integrate a high volume of transgenes in a range of dividing and non-dividing cell types. Pseudo-lentiviral particles are typically produced from 293T cells and harvested from the medium 48-78hrs post-transfection. To ensure that the pseudoviral medium is viable, and to control the number of copies of integrated viral constructs per target cell, the viral titre needs to be determined before transduction experiments. Our HIV1 p24 SimpleStep ELISA® can be used to rapidly and sensitively determine the titre of HIV1 particles in range of sample types.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

        -Single-wash protocol reduces assay time to 90 minutes or less        -High sensitivity, specificity and reproducibility from superior antibodies        -Fully validated in biological samples        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

ASSAY SPECIFICITY This kit recognizes both native and recombinant HIV-1 p24 protein in serum, EDTA and heparin plasma, cell culture supernatant, and cell and tissue extract samples only. Urine, saliva, and milk samples have not been tested with this kit.

CROSS REACTIVITY Recombinant HIV-1 Gag protein was prepared at 50 ng/mL and 1 ng/mL and assayed for cross reactivity. 1% cross-reactivity was observed.