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The buffer is one of the most popular currently used, and is commonly employed in molecular and cell biology, chemistry, and material science, among many others. 配制L Sodium Phosphate Buffer M表 1. 配方 试剂名称试剂量浓度分子式/分子量CAS号2.231 g0.018 MNaH2PO4/119.987558-80-711.555 g0.081 MNa2HPO4/141.967558-79-4在合适的容器中装入 0.8 L 的蒸馏水.往溶液中加入 2.231 g 的 磷酸二氢钠.往溶液中加入 11.555 g 的 磷酸氢二钠.加入 蒸馏水 直到体积为 1 L.Phosphate buffer is highly water soluble and has a high buffering capacity, but will inhibit enzymatic activity and precipitates in ethanol. |
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