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十一种先前未描述的木脂素成分,包括五种 8- O -4' 型新木脂素,荚蒾糖甙 A–E ( 1 – 5 ),三种苯并呋喃型新木脂素,荚蒾糖甙 F–H ( 6 – 8 ),和三种四氢呋喃型木脂素,荚蒾糖甙 I –K ( 9 – 11 ),从Viburnum urceolatum的果实中分离出来。通过对 NMR和 HRESIMS 数据的广泛分析,阐明了所有分离株的结构。通过量子化学电子圆二色性计算和比较确定了这些化合物的绝对构型。通过酸水解和单糖手性衍生物的 HPLC 分析鉴定了荚膜糖苷 A–K 的糖单元。体外酶抑制实验表明,荚莲果苷J ( 10 )对α-淀粉酶和α-葡萄糖苷酶的抑制活性最强,IC 50值分别为19.75和9.14 μM,强于阳性对照阿卡波糖。 (分别为 37.31 和 26.75 μM)。还通过分子模型分析了荚莲果苷 J ( 10 ) 与α-淀粉酶和α-葡萄糖苷酶的潜在结合模式。
"点击查看英文标题和摘要" Lignan constituents with α-amylase and α-glucosidase inhibitory activities from the fruits of Viburnum urceolatum
Eleven previously undescribed lignan constituents, including five 8-O-4′ type neolignans, viburnurcosides A–E (1–5), three benzofuran type neolignans, viburnurcosides F–H (6–8), and three tetrahydrofuran type lignans, viburnurcosides I–K (9–11), were isolated from the fruits of Viburnum urceolatum. The structures of all isolates were elucidated by an extensive analysis of the NMR and HRESIMS data. The absolute configurations of these compounds were determined by quantum-chemical electronic circular dichroism calculation and comparison. The sugar units of viburnurcosides A–K were identified by acid hydrolysis and HPLC analysis of the chiral derivatives of monosaccharides. The in vitro enzyme inhibition assay exhibited that viburnurcoside J (10) had the most potent inhibitory activity against α-amylase and α-glucosidase with the IC50 values of 19.75 and 9.14 μM, respectively, which were stronger than those of the positive control acarbose (37.31 and 26.75 μM, respectively). The potential binding modes of viburnurcoside J (10) with α-amylase and α-glucosidase were also analyzed by molecular modeling. |
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