Agriculture consolidates the process of natural reproduction and economic reproduction. Spatial pattern of agricultural region is comprehensively influenced by factors such as natural geography, economic and social development and advances in technology. Along with the changes of these influencing factors over a long period, agricultural pattern and agricultural function present regional differentiation and spatio-temporal evolution. This lays a scientific foundation for comprehensive agricultural regionalization. China is currently experiencing a new period of coordinated development of industrialization, urbanization, informatization and agricultural modernization. This paper firstly investigates spatio-temporal changes and dynamic characteristics of modern agriculture development conditions in China. Then, it puts forward the coupling measure index system of agricultural natural factors and regional function, zoning principles and scientific method of modern agricultural regionalization under the new development background. Using cluster analysis and qualitative analysis, it carries out a comprehensive research and puts forward China's modern agriculture regionalization scheme in the new era, which divides China into 15 agricultural first-grade regions and 53 agricultural sub-regions. After doing a comparative analysis with the new scheme and the previous one, which was published in 1981, the paper makes a systematic interpretation on current producing situation, regional function, spatial pattern changes and agricultural development trend of each agricultural region and the whole country at last. The results of this research provide scientific basis for strategic structural adjustment and development of modern agriculture in each region. At the theoretical level, this paper provides a research reference for the development of modern agriculture and rural geography. At the application level, it promotes integrated geography research to serve the rural revitalization and national development strategic needs in the transition period in China. The regional agriculture is a natural, economic and technological complex system, with the characteristics of complexity, dynamics and high risk. As such, based on the congnition of spatial differentiation of modern agriculture development patterns, it is necessary to deeply analyze the regional differentiation of agricultural endogenous power, agriculture and rural territorial types, to improve observational research of agricultural geographical engineering and technology, and the economic, ecological, climatic integrated effects of regional agriculture transformation, to summarize systematically regional agricultural models and regional transformation patterns, to explore the early warning mechanism of modern agricultural complex system, and to study continuously the theories and strategic decision-making for promoting agricultural modernization and rural revitalization.