Keywords:
compound Jinyinhua granule /HPLC /neochlorogenic acid /cryptochlorogenic acid /chlorogenic acid /forsythoside A /forsythoside B /baicalin
Abstract: Objective To establish an one step assay method for 10 active components (neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin)in compound Jinyinhua granules. Methods The Kromasil C18 column (250 mm×4.6 mm,5 μm) was used with the column temperature at 35 ℃ and PDA detection wavelength at 320 nm.A gradient elution with acetonitri-0.1% phosphoric acid was adopted at the flow rate of 0.8 ml/min.The injection volume was 10 μl. Results The good resolution was achieved for the 10 active components.The linear ranges of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin were 10.20-340.06,7.19-239.81,6.12-203.84,15.00-500.00,20.16-671.85,25.19-839.67,14.61-487.14,10.52-350.66,7.32-244.01,7.61-253.82 μg/ml respectively.The average recoveries (n=6) were between 97.67% and 99.00% and RSDs between 0.94% and 1.37%.The average contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin from 10 batches of preparations were 0.579 0,1.523 5,1.080 9,0.083 1,0.468 0,0.739 6,1.977 9,0.351 5,0.246 8 and 0.256 6 mg/g. Conclusion This simple and accurate method can be used as one of quality control methods for the compound Jinyinhua granules.
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