Quickly generate graphical reports of all your plots, histograms, plates, and overlays, in a variety of output formats. FlowJo v10 has added features to enhance your graphical output, check your gating strategy, and apply common formats to multiple objects!

The Layout Editor is FlowJo’s tool for creating graphical reports. Like other areas of the program, batch operations are at the heart of the Layout Editor. In the Layout Editor you can create a single layout report or apply a layout strategy to an entire sample set.

Example of creating batched layout

A聽simple graphical report can be produced by simply dragging populations from the Workspace聽into the layout editor. In the example illustrated below, the聽FCS聽file A1.fcs was added to a聽Workspace and聽a basic rectangular gate聽was created using the rectangle tool in the Graph Window. The聽gate was copied to all samples within the Workspace by dragging the new population “Lymphocytes to the “All Samples” group.

The Layout Editor is opened by clicking on the 聽icon at the top toolbar. The “Lymphocytes” population can be added to the initial blank Layout Editor by dragging the Lymphocyte population聽into the blank space in the Layout Editor. 聽The top level population can be added to the layout the same way.

By default, FlowJo graphs the data using聽the set of parameters and graph style last used when viewing the data in the workspace. 聽To make any changes to the graph, simply double click on it and uses the graph definition window that appears to make changes.

The work produced thus far is considered a Tile, a graphical piece that will serve as a template for the rest of the files, and be reproduced for each file by substituting in the data from those files when batching. 聽To batch this report across all of your samples open up the Batch band from the Layout Editor ribbon. 聽The band gives you the choice of the output formats, whether to put the graphics in rows or columns, set the number of rows or columns, and choose whether the聽plots should be filled in going across or going down. 聽Make your selections and click the orange Create Batch Button.

Output Formats

FlowJo gives you the choice of batching to:

You can also click the 聽icon at the top right of the Layout Editor as a shortcut to execute the default batch.

There are many tools available in the Layout Editor to assist you in creating a report and there are a variety of output options from which to choose. To learn about the controls, processes or tools in the Layout Editor, please select from the options below.

Ribbons and Bands

Graph Definition Dialog

Legend Properties Dialog聽

Aligning and grouping objects

Resizing page vs. resizing plots

Making Overlays

Batch Operations (overview)

Batching Overlays and Locking Control Samples

Simple Batching

Filtered Batching

Report and Image Export Options

Adjunct Histograms

Tables in Layouts

Backgating

Line Tool

Grid Tool

Statistics Table Tool

Tools

Graph Export Techniques

Sidebar

Drag-To Guides

Layout Controls

Ribbons and Tabs

Layout Editor Menus

Graph Options and Annotation

Graphic and Drawing Tools

Statistics, Tables and Equations

Export, Output, and Printing

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