YFP激发光波长和YFP的发射波长是多少？

作者：时间：2019-09-05 10:33浏览17622 次

黄色荧光蛋白（Yellow Fluorescent Protein ，YFP）可以看做绿色荧光蛋白的一种突变体，最初来源于维多利亚多管水母（ Aequorea victoria）。相对于绿色荧光蛋白，其荧光向红色光谱偏移，而这主要是由于蛋白203位苏氨酸变为酪氨酸。其更大激发波长为514 nm，更大发射波长为527 nm。

YFP激发波长为510nm，更大发射波长为527 nm

黄色荧光蛋白（Yellow Fluorescent Protein ，YFP）可以看做绿色荧光蛋白的一种突变体，最初来源于维多利亚多管水母（ Aequorea victoria）。相对于绿色荧光蛋白，其荧光向红色光谱偏移，而这主要是由于蛋白203位苏氨酸变为酪氨酸。其更大激发波长为514 nm，更大发射波长为527 nm。

美国路阳生产有多款便携式激发光源，无需荧光显微镜就能轻松观察筛选黄色荧光蛋白表达阳性。便携式YFP激发光源能直接分选YFP表达阳性的植株，能够分选愈伤组织、植株、种子、菌落等。如欲进一步了解便携式荧光蛋白激发光源，可直接联系上海路阳生物技术有限公司的销售客服。

上图为LUV-40A黄色荧光蛋白观察眼镜

上图为LUV-550A 黄色荧光蛋白拍照滤镜

Yellow Fluorescent Proteins/常用黄色荧光蛋白的激发波长和发射波长

Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.

To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.

YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.

 黄色荧光蛋白的激发波长和发射波长