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2023-10-14 08:22| 来源: 网络整理| 查看: 265

--version

show program's version number and exit

--plotType

Possible choices: lines, fill, se, std

"lines" will plot the profile line based on the average type selected. "fill" fills the region between zero and the profile curve. The fill in color is semi transparent to distinguish different profiles. "se" and "std" color the region between the profile and the standard error or standard deviation of the data.

--sortRegions

Possible choices: descend, ascend, no, keep

Whether the heatmap should present the regions sorted. The default is to sort in descending order based on the mean value per region. Note that "keep" and "no" are the same thing.

--sortUsing

Possible choices: mean, median, max, min, sum, region_length

Indicate which method should be used for sorting. For each row the method is computed. For region_length, a dashed line is drawn at the end of the region (reference point TSS and center) or the beginning of the region (reference point TES) as appropriate.

--sortUsingSamples

List of sample numbers (order as in matrix), which are used by --sortUsing for sorting. If no value is set, it uses all samples. Example: --sortUsingSamples 1 3

--linesAtTickMarks

Draw dashed lines from all tick marks through the heatmap. This is then similar to the dashed line draw at region bounds when using a reference point and --sortUsing region_length

--clusterUsingSamples

List of sample numbers (order as in matrix), that are used for clustering by --kmeans or --hclust if not given, all samples are taken into account for clustering. Example: --ClusterUsingSamples 1 3

--averageTypeSummaryPlot

Possible choices: mean, median, min, max, std, sum

Define the type of statistic that should be plotted in the summary image above the heatmap. The options are: "mean", "median", "min", "max", "sum" and "std".

--missingDataColor

If --missingDataAsZero was not set, such cases will be colored in black by default. Using this parameter, a different color can be set. A value between 0 and 1 will be used for a gray scale (black is 0). For a list of possible color names see: http://packages.python.org/ete2/reference/reference_svgcolors.html. Other colors can be specified using the #rrggbb notation.

--colorMap

Color map to use for the heatmap. If more than one heatmap is being plotted the color of each heatmap can be enter individually (e.g. --colorMap Reds Blues). Color maps are recycled if the number of color maps is smaller than the number of heatmaps being plotted. Available values can be seen here: http://matplotlib.org/users/colormaps.html The available options are: 'magma', 'inferno', 'plasma', 'viridis', 'cividis', 'twilight', 'twilight_shifted', 'turbo', 'Blues', 'BrBG', 'BuGn', 'BuPu', 'CMRmap', 'GnBu', 'Greens', 'Greys', 'OrRd', 'Oranges', 'PRGn', 'PiYG', 'PuBu', 'PuBuGn', 'PuOr', 'PuRd', 'Purples', 'RdBu', 'RdGy', 'RdPu', 'RdYlBu', 'RdYlGn', 'Reds', 'Spectral', 'Wistia', 'YlGn', 'YlGnBu', 'YlOrBr', 'YlOrRd', 'afmhot', 'autumn', 'binary', 'bone', 'brg', 'bwr', 'cool', 'coolwarm', 'copper', 'cubehelix', 'flag', 'gist_earth', 'gist_gray', 'gist_heat', 'gist_ncar', 'gist_rainbow', 'gist_stern', 'gist_yarg', 'gnuplot', 'gnuplot2', 'gray', 'hot', 'hsv', 'jet', 'nipy_spectral', 'ocean', 'pink', 'prism', 'rainbow', 'seismic', 'spring', 'summer', 'terrain', 'winter', 'Accent', 'Dark2', 'Paired', 'Pastel1', 'Pastel2', 'Set1', 'Set2', 'Set3', 'tab10', 'tab20', 'tab20b', 'tab20c', 'rocket', 'mako', 'vlag', 'icefire'

--alpha

The alpha channel (transparency) to use for the heatmaps. The default is 1.0 and values must be between 0 and 1.

--colorList

List of colors to use to create a colormap. For example, if --colorList black,yellow,blue is set (colors separated by comas) then a color map that starts with black, continues to yellow and finishes in blue is created. If this option is selected, it overrides the --colorMap chosen. The list of valid color names can be seen here: http://matplotlib.org/examples/color/named_colors.html Hex colors are valid (e.g #34a2b1). If individual colors for different heatmaps need to be specified they need to be separated by space as for example: --colorList "white,#cccccc" "white,darkred" As for --colorMap, the color lists are recycled if their number is smaller thatn the number ofplotted heatmaps. The number of transitions is defined by the --colorNumber option.

--colorNumber

N.B., --colorList is required for an effect. This controls the number of transitions from one color to the other. If --colorNumber is the number of colors in --colorList then there will be no transitions between the colors.

--zMin, -min

Minimum value for the heatmap intensities. Multiple values, separated by spaces can be set for each heatmap. If the number of zMin values is smaller thanthe number of heatmaps the values are recycled. If a value is set to "auto", it will be set to the first percentile of the matrix values.

--zMax, -max

Maximum value for the heatmap intensities. Multiple values, separated by spaces can be set for each heatmap. If the number of zMax values is smaller thanthe number of heatmaps the values are recycled. If a value is set to "auto", it will be set to the 98th percentile of the matrix values.

--heatmapHeight

Plot height in cm. The default for the heatmap height is 28. The minimum value is 3 and the maximum is 100.

--heatmapWidth

Plot width in cm. The default value is 4 The minimum value is 1 and the maximum is 100.

--whatToShow

Possible choices: plot, heatmap and colorbar, plot and heatmap, heatmap only, heatmap and colorbar

The default is to include a summary or profile plot on top of the heatmap and a heatmap colorbar. Other options are: "plot and heatmap", "heatmap only", "heatmap and colorbar", and the default "plot, heatmap and colorbar".

--boxAroundHeatmaps

By default black boxes are plot around heatmaps. This can be turned off by setting --boxAroundHeatmaps no

--xAxisLabel, -x

Description for the x-axis label.

--startLabel

[only for scale-regions mode] Label shown in the plot for the start of the region. Default is TSS (transcription start site), but could be changed to anything, e.g. "peak start". Same for the --endLabel option. See below.

--endLabel

[only for scale-regions mode] Label shown in the plot for the region end. Default is TES (transcription end site).

--refPointLabel

[only for reference-point mode] Label shown in the plot for the reference-point. Default is the same as the reference point selected (e.g. TSS), but could be anything, e.g. "peak start".

--labelRotation

Rotation of the X-axis labels in degrees. The default is 0, positive values denote a counter-clockwise rotation.

--regionsLabel, -z

Labels for the regions plotted in the heatmap. If more than one region is being plotted, a list of labels separated by spaces is required. If a label itself contains a space, then quotes are needed. For example, --regionsLabel label_1, "label 2".

--samplesLabel

Labels for the samples plotted. The default is to use the file name of the sample. The sample labels should be separated by spaces and quoted if a label itselfcontains a space E.g. --samplesLabel label-1 "label 2"

--plotTitle, -T

Title of the plot, to be printed on top of the generated image. Leave blank for no title.

--yAxisLabel, -y

Y-axis label for the top panel.

--yMin

Minimum value for the Y-axis. Multiple values, separated by spaces can be set for each profile. If the number of yMin values is smaller thanthe number of plots, the values are recycled.

--yMax

Maximum value for the Y-axis. Multiple values, separated by spaces can be set for each profile. If the number of yMin values is smaller thanthe number of plots, the values are recycled.

--legendLocation

Possible choices: best, upper-right, upper-left, upper-center, lower-left, lower-right, lower-center, center, center-left, center-right, none

Location for the legend in the summary plot. Note that "none" does not work for the profiler.

--perGroup

The default is to plot all groups of regions by sample. Using this option instead plots all samples by group of regions. Note that this is only useful if you have multiple groups of regions. by sample rather than group.

--plotFileFormat

Possible choices: png, pdf, svg, eps, plotly

Image format type. If given, this option overrides the image format based on the plotFile ending. The available options are: "png", "eps", "pdf", "plotly" and "svg"

--verbose

If set, warning messages and additional information are given.



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