东北林蛙卵油脂肪酸成分分析及其氧化稳定性研究

In order to study the components of fatty acids, polyunsaturated fatty acid enrichment process and oxidation stability in the ovum oil of Rana dybowskii, fatty acids extracted from the ovum oil of Rana dybowskiiwere detected by GC-MS. Polyunsaturated fatty acids were separated and purified by urea package, and orthogonal test was performed. The ovum oil was stored in different conditions for 0, 6, 12, 18, 24 and 30 days. In the end, the oxidation stability was determined. Results showed that 18 kinds of fatty acids were separated from the ovum oil, the total content of unsaturated fatty acids was 76.76%, and the content of polyunsaturated fatty acids was 40.25%. The optimal conditions of polyunsaturated fatty acid enrichment process were ureafat ratio of 2, inclusion temperature of -20 ℃ and inclusion time of 15 h . When ovum oil was stored at -20 ℃ in the dark, the minimal change of peroxide value was found.