SMCC 是一种蛋白质交联剂 | 您所在的位置:网站首页 › smcc交联剂保存 › SMCC 是一种蛋白质交联剂 |
SMCC SMCC 是一种蛋白质交联剂。SMCC 接合抗原耦合脾脏细胞来诱导抗原特异性免疫反应。 生物活性 SMCC is a protein crosslinker. SMCC-conjugated antigen coupled spleen cells to induce antigen-specific immune responses[1]. 体外研究(In Vitro) SMCC or sulfo-SMCC can use to conjugate keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). In the the conjugation system, 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (the final concentration of 0.1 mM) are added in PBS with final volume of 3 ml[1]. 体内研究(In Vivo) Using the SMCC-conjugation method is able to induce potent immune responses to whole protein or peptide antigens by injection of antigen-coupled syngeneic splenic mononuclear cells in mice[1]. 分子量:334.32 性状:Solid Formula:C16H18N2O6 CAS 号:64987-85-5 运输条件:Room temperature in continental US; may vary elsewhere. 储存方式 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) 溶解性数据 In Vitro:DMSO : 25 mg/mL (74.78 mM; Need ultrasonic) H2O : < 0.1 mg/mL (ultrasonic) (insoluble) 配制储备液浓度溶剂体积质量1 mg5 mg10 mg1 mM2.9911 mL14.9557 mL29.9115 mL5 mM0.5982 mL2.9911 mL5.9823 mL10 mM0.2991 mL1.4956 mL2.9911 mL*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 In Vivo:以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1.请依序添加每种溶剂: 10% DMSO 40% PEG300 5% Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution 2.请依序添加每种溶剂: 10% DMSO 90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution 3.请依序添加每种溶剂: 10% DMSO 90% corn oil Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution |
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