pBin19 载体图谱和序列

2024-07-10 03:00| 来源: 网络整理| 查看: 265

我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。

所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。

确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)

开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。

由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。

载体名称: pBin19 载体抗性: Kanamycin 载体长度: 11777 bp 载体类型: Plant Vectors 复制子: oriV 宿主: Plants 载体来源: Bevan M. 筛选标记: Neomycin/G418(Geneticin) 拷贝数: High copy number 启动子: NOS 5'引物: M13 fwd 3'引物: M13 rev pBin19 载体载体图谱 pBin1911777 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000105001100011500oriVIS1KanRtrfALB T-DNA repeatM13 fwdMCSM13 revlac operatorlac promoterCAP binding siteNOS terminatorNeoR/KanRNOS promoterRB T-DNA repeatoriVoriTtraJ pBin19 载体载体序列 Copy Sequence

下载GeneBank文件(.gb)

LOCUS pBin19. 11777 bp DNA circular SYN 01-JAN-1980 DEFINITION Binary Agrobacterium vector for plant transformation. Also known as pBIN19 or BIN19 or Bin19 or Bin 19. ACCESSION . VERSION . KEYWORDS pBin19 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11777) AUTHORS Bevan M. TITLE Binary Agrobacterium vectors for plant transformation. JOURNAL Nucleic Acids Res. 1984;12:8711-21. PUBMED 6095209 REFERENCE 2 (bases 1 to 11777) AUTHORS Frisch DA, Harris-Haller LW, Yokubaitis NT, Thomas TL, Hardin SH, Hall TC. TITLE Complete sequence of the binary vector Bin 19. JOURNAL Plant Mol. Biol. 1995;27:405-9. PUBMED 7888628 REFERENCE 3 (bases 1 to 11777) TITLE Direct Submission REFERENCE 4 (bases 1 to 11777) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "1984"; volume: "12"; pages: "8711-21" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol. Biol."; date: "1995"; volume: "27"; pages: "405-9" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..11777 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 6..616 /label=oriV /note="origin of replication for the bacterial F plasmid" mobile_element 1317..2084 /mobile_element_type="insertion sequence:IS1" /label=insertion sequence:IS1 /note="IS1" /note="prokaryotic transposable element" CDS 2276..3067 /label=KanR /note="aminoglycoside phosphotransferase" CDS 3369..4514 /label=trfA /note="trans-acting replication protein that binds to and activates oriV" misc_feature 6113..6137 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" primer_bind 6755..6771 /label=M13 fwd /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" misc_feature 6772..6828 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(6841..6857) /label=M13 rev /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 6865..6881 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6889..6919) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(6934..6955) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." terminator complement(7504..7756) /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(8149..8937) /label=NeoR/KanR /note="aminoglycoside phosphotransferase from Tn5" promoter complement(8961..9144) /label=NOS promoter /note="nopaline synthase promoter" misc_feature 9300..9324 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin 10000..10624 /label=oriV /note="incP origin of replication" oriT 11120..11229 /label=oriT /note="incP origin of transfer" CDS 11262..11630 /label=traJ /note="oriT-recognizing protein"

我们不提供该载体，载体信息仅供查询和参考。

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