LCL 8664

细胞类型：B淋巴细胞 

ATCC Number：CRL-1805 

相关疾病：淋巴瘤是否是肿瘤细胞：0 

物种来源：猕猴 

生长状态：悬浮生长 

运输方式：冻存运输 

年限：5 years 

数量：大量 

细胞形态：

淋巴样  

Designations: LCL 8664       

Depositors:  

Rangan         

Biosafety Level 生物安全等级 :2 [Cells Contain HERPESVIRUS ]         

Shipped 提供形式 :frozen         

Medium Serum: See Propagation          

Growth        

Properties:suspension        

Organism:Macaca mulatta         

Morphology:lymphoblast                 

Source:          

Disease: lymphoma         

Cell Type:  B lymphocyte;         

Cellular Products:B lymphotropic herpesvirus         

Permits/Forms:In addition to the MTAmentioned above, other ATCC and/or regulatory permits  may be required for the transfer of this ATCC material. 

Anyone purchasing ATCCmaterial is ultimately responsible for obtaining the permits.          

Please click here for information regarding the specific requirements for shipment to your location.

Age:5 years         

Gender:male         

Comments 注释 :CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard. Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium. 

About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.

Propagation:ATCC complete growth medium:  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium,Catalog No. 30-2001.  

To make the complete growth medium, add the following components to the base medium:  fetal bovine serum to a final concentration of 10%.         

Subculturing:Subcultivation

Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week 

Cultures can be maintained by addition or replacement of fresh medium. 

Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%         

References 参考文献 : 22860: Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macacamulatta) with malignant lymphoma.Int. J. Cancer 38: 425-432,1986. PubMed: 3017870