重组CD63抗体[EPR21151](ab217345)

2024-06-20 20:08| 来源: 网络整理| 查看: 265

Lanes 1-3 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/2000 dilutionLane 4 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/1000 dilution

Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µgLane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µgLane 3 : Mouse kidney lysate at 20 µgLane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 26 kDaObserved band size: 40-60 kDa why is the actual band size different from the predicted?

Exposure times: Lanes 1-3: 3 minutes; Lane 4: 5 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular masses observed are consistent with what has been described in the literature (PMID: 9890706; PMID: 9790070).

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

【本文地址】

公司简介

联系我们